Characterization of varieties biofortified in iron and zinc and local varieties of common bean

Iron and zinc are important minerals in humans in sub Saharan Africa, whose deficiency is known as "hidden hunger" due to the lack of recognised symptoms in the early stages. Although iron deficiency is the most prevalent, zinc is also involved in inhibition of replication of viruses, including the corona virus (COVID-19). In North Kivu and South Kivu provinces where more than 50% of common bean is produced and consumed in Democratic Republic of Congo, 36% and 47% of preschool children are anemic due to iron deficiency. This paradox is mainly due to insufficiency of iron-rich foods. The aim of this study is to characterise 59 iron and zinc biofortified varieties together with six local varieties of common bean for a potential selection programme in Butembo town in the Democratic Republic of Congo. We focused on 15 qualitative and five quantitative parameters. The qualitative parameters were helpful to distinguish the different morphotypes and for cluster analysis. In addition to the descriptive statistics, the quantitative data were used for Pearson correlation and for principal component analysis, PCA. Qualitative parameters enabled grouping of the study genotypes into 14 morphotypes according to the aspect and colour of the seed coat, the colour around the hilum and the size of seeds. Clustering grouped the 65 genotypes into 12 clusters with the most similar genotypes grouped in the same cluster. Quantitative parameters showed that the study genotypes were dissimilar (P=0.00). A positive correlation was obtained between the days to flowering and the days to maturity (P<0.05) and between the number of pods per plant and the days to flowering. A strong correlation was found between the number of pods per plant and seeds per pod (P<0.01). In contrast, a negative correlation was observed between the 100 seed weight and the number of seeds per pod. The PCA represented on two perpendicular axes showed 64.1% of the total variance of which the 42.3% is explained by the first axis and 21.8% by the second axis. Overall, the study genotypes are morphologically and quantitatively different and thus can be used in a selection programme.

Human activities at night negatively impact Little Penguin (Eudyptula minor) numbers and behaviours

The presence of humans within the natural environment is increasing worldwide. Assessing the impact of such activities on wildlife is crucial for declining populations where human disturbance adds to existing pressures. Here, we investigated how human activities at night influenced Little Penguin Eudyptula minor numbers and behaviours (specifically return time, number of vocalizations and time spent in vigilance) on Granite Island, a declining population in South Australia, Australia. We combined data from regular night surveys with continuous video and audio monitoring to assess the impact of human activities on the Little Penguins. The use of white light (i.e. from torches or camera flashes) by people was the most frequent activity recorded at night (recorded on 65% of the monitored nights). Fewer penguins were found on land at night when Dogs Canis lupus familiaris were present, but not when the number of people increased, when concerts occurred, or when white lights were used. Little Penguins were observed more often returning late from sea at night when Dogs were present and when white lights were used, but not when concerts occurred. An increase in penguin vocalizations at night correlated with the presence of Dogs and the occurrence of concerts, whereas penguins vocalized less when white lights were used. The time Little Penguins spent in vigilance did not correlate with any of the disturbances analysed. Our study also highlights the impact of coronavirus disease 2019 (COVID-19) on wildlife, as the occurrence of human activities increased significantly following the implementation of the COVID-19 health protection measures. These results add to a growing body of literature suggesting that human activities on land, and their consequent disturbance(s), may affect the numbers and behaviours of wildlife and that appropriate measures need to be developed to limit such impacts.

Re-evaluating the precision of yield targets set by MOET app v.2.0

This paper presents the evaluation results of the yield target setting precision of the revised MOET App (v.2.0), following the inclusion of the correction factors generated from rice biomass correlations between MOET and nutrient omission plot setups obtained from 2017 to 2018. The project started with trainings on MOET kit and MOET App use for the seed production personnel across PhilRice stations in Nueva Ecija, Negros, Bukidnon and Agusan in 2018 DS. Included in the trainings were the establishment of MOET kit tests and generation of variety- and site-specific recommendations via the MOET App for several nationally or regionally recommended varieties (NSIC Rc 122, 160, 216, 218, 222, 238, 286, 300, 358, 402, 436, 440, 442, 480, PSB Rc18 and PSB Rc82) that each PhilRice station intended to produce in the succeeding 4 cropping periods from 2019 to 2020. Relative yield advantages and economic benefits from using the MOET App fertilizer recommendations over PhilRice' current fertilizer management in seed production per station were monitored every cropping while the precision evaluation of yield target setting was done after the last cropping of 2020 WS. In 2019, relative yield advantages averaged 0.43t ha-1 in DS and 0.25t ha-1 in WS. In 2020 DS, an average relative yield advantage of 0.63t ha-1 was obtained across stations and 0.93t ha-1 in 2020 WS in Nueva Ecija only due to travel restrictions brought about by the COVID-19 pandemic. Economic benefits of using the MOET App showed an average of 0.50t ha-1 and 0.65t ha-1 yield increase over the seed production units' fertilizer management in DS and WS, respectively. While savings in fertilizer cost were better realized during the WS at an average of Php 4,126.34 ha-1 season-1 across stations. Results of the precision evaluation of the yield target showed marked improvements with a 95.24% probability of achieving 17% higher grain yields than the target set by MOET App v.2.0. However, the overall normalized Root Mean Square Error (nRMSE) of 38.14% exceeded the range for a fairly acceptable fit with the model due to large gaps between target and actual yields obtained from DS field trials.

Swine breeding in the villages of Valcea county, Oltenia (Romania) - tradition or necessity?

Food supply has been a constant source of concern for mankind. In the present context, with food security a priority of European and national policies, an analysis of pig farming in a representative NUTS2 administrative level of Romania that emphasizes the proportion of households raising at least one pig and the main factors influencing farmers to adopt or give up swine breeding could allow a much clearer understanding of this phenomenon that lies at the border between cultural tradition and socio-economic necessity. This study uses mixed methods that complement each another to help reveal this complex phenomenon in the analyzed territory. Cluster analysis shows the concentration of swine breeding and maps its spread in terms of both subsistence and larger farms, and qualitative interviews prove the motivation of farmers to continue in this occupation. As a primary result, the study visualizes the spatial distribution of pig farming in the rural environment of Valcea county, Romania, from a diachronic perspective in the post-communist period. It also reveals areas of differing concentrations of both very small-sized farms, which prioritize meeting their own food needs, and larger farms, which prioritize commercial production to supplement their revenue streams. Both categories, but particularly the latter, are of particular interest in a period in which the socio-economic environment after 1990 - marked by economic restructuring, unemployment, population migration, the economic crisis of 2008-2010, the pandemic of 2020-2021, and the most recent energy crisis - periodically highlights the importance of rural areas in ensuring food security and sufficiency at both the local and regional levels.

Preface

The 2nd Agrifood System International Conference (ASIC)Professor Jurnalis Kamil Convention Hall, Padang, West Sumatra, Indonesia, 8-9 November 2022"Research advancement and innovations in agroecology and smart agrifood systems.”The 2nd Agrifood System International Conference (ASIC 2022) was successfully held on 8-9 November 2022. Due to the covid-19 pandemic, this event was held virtually via the zoom platform, directly from Professor Jurnalis Kamil Convention Hall, Padang, West Sumatra, Indonesia. This event was organized by the Faculty of Agriculture, Universitas Andalas, Indonesia, and became a part of the event to commemorate the 68th anniversary of the faculty. The theme of the ASIC 2022 was: "Research advancement and innovations in agroecology and smart agrifood systems.”There have been numerous revolutions in agriculture, which have improved competency and led to record-breaking yields and gains. The latest process is "smart farming,” contributing to humanity's survival and future prosperity. Smart farming presents numerous prospects for pervasive interconnection and database computer technology as part of Industry 4.0. Smart farming is the idea of agricultural practice in a creative manner while utilizing cutting-edge technology to improve the quantity and quality of agricultural goods. New methods to assure global food safety are part of the future of the food manufacturing industry. It enables farmers to boost yields more effectively and efficiently. Fertilizers, labor, seeds, and water are just a few resources that can be saved. Smart farming has supporting applications, including land management, selection of varieties, minimizing synthetic fertilizers and pesticide inputs, and replacing them with environmentally friendly inputs. Research and related technological innovations have been carried out but have yet to be adopted and properly integrated.The main objective of this conference was to provide a venue for exchanging knowledge, scientific advancement, and innovative ideas among researchers, academicians, governments, and organizations. The scope includes plant breeding and crop production, soil management, plant protection and food safety, the socio-economic of agriculture and natural resources, and all topics related to agriculture. The committee received more than two hundred paper s coming from 46 institutions, national and international. We encourage student presenters from undergraduate to doctoral programs to present their papers;hence, around 25% of s come from them.The conference program was divided into two main segments: plenary and parallel. The plenary session invited 13 speakers from within and outside the country and was attended by 610 participants during the two days' activities. On behalf of the committee, we greatly appreciate the seven speakers contributing and sharing their knowledge at this event: Dr. Silvain R Perret, Scientific Director of CIRAD, France;Mr. Pierre Ferrand from FAO, Regional Office for Asia and the Pacific;Prof. Norman Uphoff, SRI Scientist from Cornell University, USA;Dr. Jauhar Ali, Rice hybrid breeder from IRRI, Philippines;Dr. Trevor A. Jackson, Plant protection scientist from IAPPS/ Coordinator Region XII;Prof. Shamshuddin Jusop, Soil Science Scientist from UPM, Malaysia;and Dr. Wahono: Drone creator from UMM, Indonesia. We also introduced five invited speakers from the Faculty of Agriculture: Dr. Irawati Chaniago - Crop Production;Dr. Dini Hervani - Plant Breeding;Dr. Eka Candra Lina - Plant Protection;Dr. Yuerlita - Socio-economics of Agriculture;Dr. Hery Bachrizal Tanjung - Agricultural Extension. In addition, we have provided an online workshop conducted as a side event on successfully publishing an article in IOP-EES Proceeding.Finally, let me express my sincere gratitude to all presenters, participants, and committee members who contributed significantly to this event's success. Special thanks go to the Rector of Universitas Andalas and the head of the research institute and community service of Universi as Andalas for all the support during the event. We hope to deliver the 3rd ASIC in 2024.Warmest regards,Dr. My SyahrawatiChairperson of the Organizing CommitteeList of Documentations, Conference Committee, Conference Schedule, Parallel Schedule, List of Presenters are available in this Pdf.

Adoption pattern of 448 varieties of tomato among the farmers of Ananthapuramu District

Andhra Pradesh is one of the major tomato producing states in the country. The presents tudy was conducted to identify the adoption pattern of 448 variety of tomato in Ananthapuramu district of Andhra Pradesh which was released by IIHR, Bangalore. The study also includes identification of the major factors for adoption and find out the major attributes of innovation that led to the adoption of 448 variety. An ex-post facto research design was adopted for the study. The study included 40 respondents chosen by purposive sampling method. Data was collected with the help of well-structured interview schedule through telephonic interview due to COVID-19 pandemic. The statistical tools used for analysis are mean, standard deviation, cumulative frequency and percentage analysis. Profile characters of the respondents viz., age, educational status, occupation, farm size, farming experience, annual income, frequency of exposure to agricultural messages, scientific orientation and cropping pattern were studied. Based on the mean and SD values the respondents were classified into adopter categories. The results of the adopter categorization showed that two-fifths (40.00%) of respondents belonged to early majority and only 5.00 percent were innovators. Among the factors affecting adoption of 448 variety of tomato, majority (80.00%) adopted due to their own interest and with the influence of friends and neighbours. Compatibility (95.00%) and observability (82.50%) of the variety played an important role among the attributes of innovation for the adoption of 448 variety of tomato among the respondents.

Utilization of Waste Banks System in the COVID-19 Pandemic

People's perspectives and behaviors altered in a variety of ways during the pandemic period, most notably in areas related to health, environment, and most notably waste management. Many people still prefer to work from home and this in turn leads to a large accumulation of garbage without having any proper means of disposal. As a result, the garbage area will serve as a breeding ground for illness. Garbage is usually composed of a residual waste generated from a product or object that has outlived its useful life, but can still be recycled into a valuable item. On this basis, the solution is to use a waste bank;it is a collection point for sorted garbage. It is claimed that using a waste bank can help in preventing the garbage accumulation. The main purpose of this study is to determine the contribution of the waste bank to end garbage accumulation. The information gathered through a review of the literature and direct interviews with multiple informants. According to the findings of the literature review, waste banks can provide a variety of benefits to users, as well as the community and surrounding environment. The proposed research study's findings show that the changes in community activity patterns contribute to an increase in garbage, and that the community plays an important role in waste control. From the performed research study, it is evident that m any individuals are interested about garbage banks and their benefits based on this presumption. © 2023, The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.

Exploration on the potential mechanism of baicalein on Porcine Deltacoronavirus infection based on network pharmacology

This study aimed to explore the protective mechanism of baicalein against porcine deltacoronavirus (PDCoV) infection. The targets of baicalein were obtained through Pharmamapper, Pubchem, STITCH, TCMSP and Swiss Targer Prediction databases, and the targets of PDCoV infection were obtained according to the proteomics data from our previous study. The targets of baicalein-PDCoV interaction were obtained and analyzed by STRING database and Cytoscape 3.8.2 software to construct a network diagram of "baicalein-PDCoV-targets". The CytoNCA was used to analyze network topology and core network construction. Metascape database was used for GO and KEGG analysis of core network genes. The expression levels of genes in the predicted signaling pathways were detected in vitro. A total of 268 potential targets of baicalein were screened out. There were 75 potential targets of baicalein-PDCoV infection. GO enrichment results showed that baicalein was mainly involved in the formations of membrane raft, spindle and mitochondrial membrane, cell cycle and MAPK signaling pathways. A total of 277 signaling pathways (P < 0.01) were screened out by KEGG enrichment. The PI3K-Akt, Ras and MAPK signaling pathways were the main pathways that involved in the protective effects of baicalein against PDCoV infection. The results showed that compared with the cellular control groups, the mRNA expressions of PI3K, AKT and NF-B significantly increased in the PDCoV infection group. Compared with the PDCoV group, treatment of baicalein significantly decreased the mRNA expressions of PI3K, AKT and NF-B (P < 0.05). The effect of baicalein on PDCoV infection has the characteristics of multi-targets and multi-pathways, through the intervention of AKT1, HSP90AA1, SRC, EGFR, CASP3, MAPK, STAT3 and other core genes in regulating PI3K-Akt signaling pathway, Ras signaling pathway and MAPK signaling pathway, apoptosis, and virus infection. These results suggested that baicalein could be a potential therapeutic drug against PDCoV infection for further study.

Wild goose chase: Geese flee high and far, and with aftereffects from New Year's fireworks

In the present Anthropocene, wild animals are globally affected by human activity. Consumer fireworks during New Year (NY) are widely distributed in W-Europe and cause strong disturbances that are known to incur stress responses in animals. We analyzed GPS tracks of 347 wild migratory geese of four species during eight NYs quantifying the effects of fireworks on individuals. We show that, in parallel with particulate matter increases, during the night of NY geese flew on average 5–16 km further and 40–150 m higher, and more often shifted to new roost sites than on previous nights. This was also true during the 2020–2021 fireworks ban, despite fireworks activity being reduced. Likely to compensate for extra flight costs, most geese moved less and increased their feeding activity in the following days. Our findings indicate negative effects of NY fireworks on wild birds beyond the previously demonstrated immediate response.

Ukraine's market of certified seed: current state and prospects for the future

The production of high-quality seeds and planting material is the basis for increasing the efficiency and sustainability of crop production. The main aim of this article is to develop proposals to ensure that supply meets the demand in the seed and planting material market Ukraine. The future prospects are also discussed. The paper uses statistical and comparative analysis. The patterns of foreign trade in seeds and planting material to and from Ukraine are analyzed. The high level of import dependence of Ukraine leads to excessive exposure to instability in the world seed market. The development of seed production in Ukraine is discussed and analyzed along with the ways for improving commercial circulation of seeds and planting materials. The export volumes of grains and oilseeds in 2020 were the highest of those over the last three decades and amounted to USD 18.7 million, which is almost twice more than in 2019. Corn seeds dominate in exports (72%). The volume of imports of seeds of grains and oilseeds exceeded exports by 22 times in value and, in 2020, amounted to USD 409.4 million. In the total volume of imports, imports of sunflower seeds accounted for 53%. The upward trend of seed imports has been maintained since 2010. It was the result of increased demand for imported seeds by large- and medium-sized agricultural producers. In 2020, COVID-19 gave impetus to the development of domestic seed production and foreign breeding companies within the country. Prospective ways to accelerate the development of the organization of the Ukrainian seed and planting material market are outlined. Ukraine has prospects for increasing the export of seeds of grains and oilseeds by expanding its production by foreign companies operating in Ukraine. Solving problems of competitiveness seed production in Ukraine will make it possible to strengthen the role of domestic breeding in the seed market, as well as to use the best foreign varieties through their commercial circulation.

Evaluation of Indonesian Butterfly Pea (Clitoria ternatea L.) Using Stability Analysis and Sustainability Index

Yield and yield attributes are important components in genotypic evaluation. The butterfly pea is a native plant of Indonesia, and it is considered an underutilized crop. The goals of this study were to evaluate genotypes using environment (year) interactions (GEIs) with yield and yield attributes, and evaluate butterfly pea genotypes based on stability measurements and sustainability index (SI). The study was conducted at the Ciparanje Experimental Field, Faculty of Agriculture, Universitas Padjadjaran using 35 butterfly pea genotypes in a randomized complete block design with two replications. The field trial was conducted over three years (2018–2020). The results showed that the yield and yield attributes were influenced by GEIs. Additive main effects and multiplicative interaction (AMMI) selected 11 stable genotypes (31.43%);genotype plus genotype by environment interaction (GGE) biplot, AMMI stability value (ASV), and genotype stability index (GSI), each selected six genotypes (17.14%) that were stable and high-yielding, and SI selected 18 genotypes (51.43%) that were stable and high-yielding. There were three genotypes identified by all measurements, namely G2, G14, and G16. These three genotypes can be selected as the superior genotypes of the butterfly pea for flower production, and can be used as material for crosses in plant-breeding prog.

Brachycephalic obstructive airway syndrome: an overview

One of the conformational issues by the explosion Of pet ownership throughout the COVID 19 pandemic is the Ming number dunes of brachycephalic obstructive envay syndrome (BOAS), which is a condition prevalent in some of the UK's most copular dog breeds The challenges the veterinary profession is not only to identify and treat affected individuals from within the population of dogs presenting to primary care clinicians, using surgical and non-surgical options but also to educate clients on how to recognise clinical signs of the disease as early as possible.

Selection and evaluation of environmentally friendly pesticides, biological preparations and resistant variety samples against harmful objects of gourds in the conditions of the south-east of Kazakhstan

In the Republic of Kazakhstan melons and gourds are produced mainly in the traditional way, that is, by using chemical fertilizers and pesticides. The production of environmentally friendly melons is at the initial stage of its development. There is no organic melon growing at all. Considering the huge export potential of melons among other crops cultivated in the country (the total supply of melons and melons on the domestic market is 472%), the great economic and social significance of the transition of the melon industry to organic production is growing. In the future, Kazakhstan may be in the center of attention of the world community as a manufacturer and supplier of environmentally friendly (organic) melons, and environmentally friendly, natural melons may well become a brand of our sovereign state. To do this, it is necessary to make fundamental changes in the melon industry of the country. In general, the transition of agricultural production to an organic direction will contribute to the consumption of the safest and most useful products by the local population. Here, one should also take into account the decrease in the immunity of the human body due to various diseases, including those associated with the coronavirus pandemic. It should also be taken into account that only a limited number of pesticides used against harmful objects in agriculture act as intended, and most of the toxic substances spread into the environment. The use of disinfectants without scientific support has a negative impact on flora and fauna, being deposited in the soil and irrigation system of fields for a long time, thereby causing enormous damage to the environment. Given the above facts, our scientific research was aimed at the selection of pesticides and biological preparations with high biological and economic efficiency, low rates and frequency of treatments, as well as varieties with high resistance to fungal diseases of melons (watermelon, melon). Research work was carried out on the experimental plots of the Regional Branch "Kainar" of the LLP "Kazakh Research Institute of Horticulture" in the period 2020-2021. The studies used classical methods generally accepted in melon growing. The results obtained are new and relevant, and in the future, will contribute to the transition from traditional melon growing to biological.

STING gene knockout pig alveolar macrophage cell model constructed by CRISPR/Cas9 technology and its effect on type I interferon transcription. (Special Issue: Swine industry science.)

Objective: To elucidate the mechanism of interferon gene stimulating factor (STING) in the anti-pathogenic microbial infection of pigs, so as to further provide a reference for the scientific prevention and control of viral diseases such as porcine transmissible gastroenteritis, epidemic diarrhea and porcine pseudorabies. Method: High-scored targets were found in exons 4 and 8 of STING gene and corresponding sgRNA sequences were designed based on CRISPR/ Cas9 technology. The annealed sgRNAs were linked with the enzyme digested LentiCRISPRV2 carrier with T4 DNA ligase to obtain LentiCRISPRV2-STING-sgRNA lentivirus carrier(STING-sgRNA);Different combinations of STING sgRNA lentivirus carriers, packaging plasmid psPAX2 and envelope plasmid pMD2.G were transfected into 293T cells to obtain lentivirus containing sgRNA and then transduced into 3D4/21 cells. Monoclonal cell lines were obtained by puromycin screening and limited dilution method. The knockout efficiencies of the STING gene were identified by PCR amplification, Sequencing and Western blotting;The effect of STING gene knockout on the expression of type I interferon was verified by real-time fluorescent quantitative PCR. Result: When 293T cells were transfected with different combinations of STING-sgRNA lentivirus carrier and HA-STING over expression vector, the editing effect of STING eukaryotic expression carrier could be detected in cells, and the combination of STING-sgRNA(1+5)lentivirus carrier showed the supreme editing efficiency. Thus, the STING-sgRNA(1+5)lentivirus carrier combined with the packaging plasmid psPAX2 and the envelope plasmid pMD2.G were transfected 293T cells to package lentivirus, and then infected 3D4/21 cells with lentivirus. The results showed that a 3D4/21 cell line with a large deletion of the STING gene(4989 bp)was obtained. The STING protein was not observed by Western blotting, indicating that the STING gene knockout 3D4/21 cells(3D4/ 21-STING-/-)were successfully constructed. The transcription level of IFN-beta in 3D4/21-STING-/- cells decreased significantly (P<0.05) compared with parental cells when stimulated by transfection of Haemophilusparasuis DNA. Conclusion : By applying CRISPR/Cas9 technology, STING gene is successfully knock out in 3D4/21 cells, resulting in loss of function of STING gene;STING knockout leads to the transcription disorder of type I interferon when cells are stimulated by DNA, which also suggests that STING gene may be a key factor in the anti-pathogenic microbial infection of pigs.

An inaugural survey of cereal, oilseed and vegetable crop diseases in the Yukon Territory in 2020

Various kinds of field crops growing on two commercial farms in the Whitehorse area of the southern Yukon Territory were surveyed for diseases in summer 2020 by staff of the Agriculture Branch of the Government of Yukon. They included barley, wheat, canola, beets, broccoli, cabbage, carrots, potatoes and turnips. Fields were visited one or more times during July and August. The incidence and severity of diseases were visually assessed on a crop-by-crop basis and samples were collected for laboratory analysis of the pathogens present, if any. Both infectious and non-infectious diseases were present on most crops. The infectious diseases were caused by various species of plant pathogenic bacteria and fungi that were common on these crops growing in other areas of Canada. INTRODUCTION AND METHODS: The 2020 field crop disease survey is believed to be the first organized study of its kind on agricultural crops in the Territory. In his book, "An Annotated Index of Plant Diseases in Canada . . . ", I.L. Conners lists over 300 records of plant diseases on trees, shrubs, herbs and grasses in the Yukon that were published by individuals who were surveying forests and native vegetation mainly for federal government departments, universities and other agencies (Conners 1967). The objectives of the 2020 survey were: (1) to determine the kinds and levels of diseases on selected Yukon crops, (2) to identify the major pathogen species attacking Yukon crops, and (3) to use the results to plan future surveillance activities aimed at helping producers to improve their current disease management programs. All of the fields included in the 2020 survey were situated on two commercial farms, which were designated as Farm #1 and #2, in the Whitehorse area in the southern Yukon (Fig. 1). The crops surveyed included cereals (barley and wheat), oilseeds (canola) and vegetables (beets, broccoli, cabbage, carrots, potatoes and turnips). Fields were visited one or more times in the mid- to late growing season (July/August) at a time when damage from diseases was most noticeable. Symptoms were visually assessed on a crop-by-crop basis by determining their incidence and severity. Incidence was represented by the percentage of plants, leaves, heads, kernels, etc., damaged in the target crop, while severity was estimated to be the proportion of the leaf, fruit, head, root/canopy area, etc., affected by a specific disease as follows: Proportion of the canopy affected based on a 0-4 rating scale, where: 0 = no disease symptoms, 1 = 1-10% of the crop canopy showing symptoms;2 = 11-25% showing symptoms, 3 = 26- 50% showing symptoms, and 4 = > 50% showing symptoms. Photographs of affected plants were taken and sent to plant pathologists across Western Canada for their opinions on causation. Where possible, representative samples of plants with disease symptoms were packaged and sent to the Alberta Plant Health Lab (APHL) in Edmonton, AB for diagnostic analyses. Background information, such as the general cultural practices and cropping history, was obtained from the producers wherever possible. GPS coordinates were obtained for each field to enable future mapping Cereals: Individual fields of barley (11 ha) and wheat (30 ha) located at Farm #1 were surveyed. The barley was a two-row forage cultivar 'CDC Maverick', while the wheat was an unspecified cultivar of Canada Prairie Spring (CPS) Wheat. Plant samples were taken along a W-shaped transect for a total of five sampling points for the barley field (< 20 ha) and ten sampling points for the wheat field (> 20 ha). The first visit, which occurred on July 30, involved visual inspection and destructive sampling wherein plants were collected and removed from the field for a detailed disease assessment at a lab space in Whitehorse. There, the roots were rinsed off and the plants were examined for disease symptoms. The second visit to these fields, which occurred on August 27, only involved visual examination of the standing crop. Oilseeds: A single 40 ha field of Polish canola (cv. 'Synergy') was examined o

Agricultural biotechnology for sustainable food security.

Of late, global food security has been under threat by the coronavirus disease 2019 (COVID-19) pandemic and the recent military conflict in Eastern Europe. This article presents the objectives of the Sustainable Development Goals and the European Green Deal related to achieving food security and sustainable development in European Union (EU) agriculture, taking the aforementioned threats into account. In addition, it discusses the future of plant agricultural biotechnology and artificial intelligence (AI) systems, considering their potential for reaching the goal of food security. Paradoxically, the present challenging situation may allow politicians and stakeholders of the EU to realize opportunities and use the potential of the biotechnology sector.

BIODIVERSITY, WHAT KIND OF RESEARCH TO FORSEE RISKS?

Biodiversity combines species, their genetic variability, their ecosystems and all the relationships between them. Endosymbiosis is one of the more important, the probable way between prokaryotes and eukaryotes. Evolution of life relies on combining previous elements into new ones. The discovery of microbiome changes the way microorganisms are considered within the whole biodiversity and for their links with sanitary challenges. Cooperation seems as important as competition. Is it then pertinent to see the development of genomics as the only possibility to discover new viruses in reservoir species before they start new pandemics and to prevent them? Is it the good way to ask the question of new sanitary risks emergence? What about considering the ecology of epidemiological cycles? Or working breeding practices with more connection to local biodiversity? How human sciences could be taken into account? A few examples will illustrate some of these points. Copyright © 2022 Academie Veterinaire de France. All rights reserved.

Leguminous crop proteins. (L'Accademia per il post Covid-19.)

In our Country, protein crop have undergone a significant reduction since the beginning of the new millennium. Such a drastic reduction in planted areas has led, from an agronomic-environmental point of view, to an unsustainable simplification of crop rotations with serious consequences on the typical crop systems of Mediterranean agriculture. On the contrary, access to the agri-environmental subsidies requires crop rotation for farms requesting access to supplementary aid within the Rural Development Plans. It is worth mentioning that protein crops are also very suitable for "low input" itineraries. For instance, soybeans do not require specific nitrogen fertilizer, while other species perform well with minimum tillage, while others (rapeseed) with new rapid-growth hybrids, are able to assimilate important quantities of nitrates while preserving the rhizosphere from the common nutrient-loss problems. It therefore appears essential to reduce this heavy state of dependence, on the one hand by regaining space for the growing of protein crops, in particular soybean, which has undergone a significant reduction, and on the other, through a diversification of vegetable protein sources.

The development of beef cattle breeding business at Anutapura Public Animal Husbandry school during the COVID-19 pandemic and post earthquake, tsunami and liquefaction in Sigi Regency, Central Sulawesi, Indonesia

Earthquake is the vibrations on the earth's surface due to the sudden release of energy from the earthquake center in the earth. The released energy propagates through the ground in the form of vibrations. It is a natural ground movement caused by a variety of phenomena, including tectonic processes, volcanism, and explosions, as well as collapse. An earthquake with a magnitude of 7.4 on the Richter Scale that rocked the Central Sulawesi region caused the tsunami that hit the Talise Beach and the liquefaction in Petobo and Balaroa of Palu City, as well as the liquefaction in Sibalaya of Sigi Regency. After the disaster, the Sigi area, especially Bulubete Village, was the subject of flash floods due to the changes in waterways which should pass into the residential area instead through the river. This study aimed to determine the Development of Beef Cattle Breeding Business at the Public Animal Husbandry School (SPR) in Sigi Regency during the Covid-19 pandemic as well as the earthquake, tsunami, and liquefaction. This research was conducted from March to May 2022. The location of this research was determined based on purposively. This research was conducted in Bulubete Village of Sigi Regency. Population method was used in determining the number of samples which was also used as the sample of 36 breeders. The result obtained in this study was that natural disasters and the spread of covid-19 greatly affected the development of the cattle business at Anutapura SPR of Bulubete Village of South Dolo District of Sigi Regency. The population and demand at Anutapura SPR experienced a sales trend after the disaster and the covid-19 pandemic. In this case, the cattle population increased before the natural disaster and decreased after the natural disaster coupled with the covid-19 pandemic which affected the demand for beef.